WNT proteins have diverse roles in governing cell fate, proliferation, migration, porarity and death.
WNT signaling controls development and is important for embryogenesis and carcinogenesis (1-3).
WNTs are transduced through at least three distinct intracellular signaling pathways including the canonical ‘WNT/b-catenin’, ‘WNT/Ca2+’ and ‘WNT’ polarity (also called the ‘planar polarity’ pathway) (2,4,5) pathways.
LRP5 (Low Density Lipoprotein Receptor-related Protein 5) is a member of the LDL receptor family, binds apolipoprotein E, and is widely expressed in many tissues, including hepatocytes, adrenal gland, and pancreas (6).
LRP5 functions as a WNT co-receptor and interacts with Frizzled receptors.
LRP5 is important in embryonic development, bone accrual development, eye development, normal cholesterol metabolism, and glucose-induced insulin secretion (6).
Expression of LRP5 has also been used as a novel marker for disease progression in high-grade osteosarcoma (7).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 2-3ug/ml; Note: For optimal results in immunofluorescence assays, cells should be fixed in 4% paraformaldehyde/0.5% Triton X-100/PBS for 10 minutes, permeabilized with 0.5% Triton X-100/PBS for 5 minutes, and blocked with 5% BSA/PBS for 30 minutes
Western Blot: 1-3ug/ml; Single band at ~200kDOptimal dilutions to be determined by the researcher.
Positive Control:Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.
