The Wnt family of secreted glycoproteins mediate cell-cell interaction during cell growth and differentiation in both embryos and adults.
(1,2) Canonical Wnt signaling by way of the b-catenin pathway is transduced by two receptor families, frizzled proteins and lipoprotein-receptor-related proteins 5 and 6 (LRP5/6) which binds Wnts and transmit their signal by stabilizing intracellular b-catenin.(3-6) Kremen1 and Kremen2 are high-affinity Dkk1 receptors that functionally cooperate with the secreted protein Dickkopf1 (Dkk1) to block Wnt/b-catenin signaling.(7) Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signaling through LRP6 in vertebrates.(7) Dkk1 has been shown to inhibit Wnt signaling by binding to and antagonizing LRP5/6 (8-10).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human, mouse and rat Kremen2 proteins at ~51kD. No cross-reactivity with the related Kremen1 protein. Reactivity has been confirmed with myc-tagged mouse Kremen2-transfected 293T cell lysates. Species sequence homology: human and rat-100%.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the internal region of human, mouse and rat Kremen2 proteins.
