The human Dickkopf (Dkk)-related proteins are a structurally and functionally heterogeneous family of proteins.
This family is comprised of hDkk1, -2, -3, -4 and Soggy (Sgy), a Dkk-3 related protein.
Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues and are secreted when expressed in 293T cells.
Several secreted antagonists, including Dickkopf-1 (Dkk-1) (German for “big head, stubborn”) regulate the activity of Wnt proteins secreted glycoproteins that influence many aspects of embryonic development in metazoans.
Dkk-1 is a head inducer secreted from the vertebrate head organizer, that induces anterior development of Xenopus by antagonizing Wnt signaling.
Dkk-1 inhibits Wnt-induced axis duplication, acts upstream of disheveled3 and antagonizes Wnt signaling upstream of its effect on beta-catenin regulation.
Dkk-1 is an LRP6 ligand5 that inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation.
Dkk-1 plays a crucial role in the control of programmed cell death during vertebrate limb development.
Bmp-4 is one of several upstream regulators of Dkk-1 expression.
Furthermore, the proapoptotic hDkk-1 gene may play important roles in linking the oncogenic Wnt and p53 tumor suppressor pathways.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human and mouse Dkk-1 proteins. On Western blots, it identifies the target band at ~35kD. Reactivity has been confirmed with human Dkk-1-transfected 293T cell lysates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from the C-terminal region of the human and mouse Dkk-1(Dickkopf-1) proteins.
