Neuromedin U (NmU) is synthesized from a large precursor peptide and cleaved into 25aa (human NmU, 25aa; rat NmU, 23aa; porcine NmU, 25aa) and 8aa (Nmu-8; 18-25) biologically active peptides.
NmU peptides from various species share the greatest homology in the their C-terminal regions, which is also critical in biological activity.
NmU is present in nerves throughout the GI-tracts, corticotrophs within the anterior and lobe of rat and human pituitary glands, parafollicular cells of in rat thyroid gland, and in various regions of brain (spinal cord, hypothalamus, substantia nigra, hippocampus, amygdala).
Low levels of NmU are also found in human adipose tissue, lymphocytes, and spleen.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: ~1:100,000 using 50-100ng control peptide/well.
Full length rat 23-aa NmU human 25-aa and mouse 23-aa NmU are also available to study antibody reactivity by ELISA
Optimal dilutions to be determined by the researcher.
Control Peptide:N2171-80J: Neuromedin U, Rat Control Peptide (NmU)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat Neuromedin U.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic 14aa peptide corresponding to rat NmU within the N-terminal domain (KLH). Species Sequence Homology: The 14aa peptide shares no significant sequence homology with human, mouse or NmU from other species.
