Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：The three individual control immunogenic NmU peptides do not share significant sequence homology with each other. Since the antibodies are made to a mixture of NmU peptides, the antibodies have reactivity with mouse, rat and human NmU as confirmed by ELISA using full length NmU from the respective species. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking experiments (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity. Full length rat 23-aa NmU, human 25-aa and mouse 23-aa NmU are also available to study antibody reactivity by ELISA.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Three peptides of human, rat and mouse origin within the N-terminal regions of NMU were synthesized and coupled to KLH.
