GLP1, a processed active peptide of 30aa (chr 2q36-q37) is a potent insulin secretagogue, plays a major role in the enteroinsular axis, accounting for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
The so-called gluco-incretin
Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide.
The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2.
The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis.
The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration.
Applications:Suitable for use in ELISA.
Other potential application, though not tested is Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100 of control peptide/wellOptimal dilutions to be determined by the researcher.
Control Peptide:G2040-36B: Glucagon-like Peptide 1, CT, Control Peptide (GLP1)
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human GLP1. No significant sequence homology is detected with other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 9aa from the C-terminal of human Glucagon-like Peptide 1 (GLP1) conjugated to KLH. Cellular Localization: Cytoplasmic. Species Sequence Homology: mouse, rat, guinea pig, bovine and porcine, 100%
