Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions.
Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Application: Suitable for use in neutralization, ELISA, and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.2-0.4 ug/ml (using 100ul/well antibody solution) Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hTNF-a.
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-a is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Reconstitution:The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA.
Storage and Stability:The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA.
This solution can be stored at 4°C for at least one month without detectable loss of activity.
Frozen aliquots of this solution are stable for at least 6 months when kept at -20°C.
Avoid more than one freeze-thaw cycle.
Country of Origin: USA
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water. Labeled with Biotin.
