UDP-glucose:glycoprotein glucosyltransferase (UGGT) is a 170kD lumenal endoplasmic reticulum (ER) resident protein that catalyzes monoglucosylation of high mannose oligosaccharides (1).
UGGT catalyzes the transfer of glucose from UDP-glucose to Man7-9GlcNAc2-moiety of unfolded glycoproteins in a Ca2+ dependent reaction (2, 3).
UGGT binds unfolded glycoprotein via two elements on its substrate: a N-acetylglucosamine unit on the oligosaccharide and hydrophobic amino acids that are exposed in the denatured conformation (4).
It is postulated that UGGT serves as a sensor that is able to distinguish between folded and denatured protein in the ER.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot (Colorimetric): 1:100 Western Blot (ECL): 1:500Optimal dilutions to be determined by the researcher.
Positive Controls:Rat Brain Tissue Extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes a 170kD protein, corresponding to the apparent molecular mass of UGGT. Species Crossreactivity: mouse and rat cell lysates and tissue extracts.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to a portion of rat UGGT.
