84-1057-63　Ubiquitin 50ug　U1000-01E

特徴

• Ubiquitin is a 76-amino acid polypeptide expressed in all eukaryotic cells and it is highly conserved from yeast to humans.
• The modification of proteins by chains of ubiquitin has long been known to mediate targeting of cytosolic and nuclear proteins for degradation by proteasomes.
• Ubiquitination is an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s).
• E1 activates ubiquitin in an ATP-dependent manner with the formation of a thiol-ester linkage between the carboxy terminus of ubiquitin and E1.
• Sequential, transient thiol-ester bonds are then generated between the carboxyl terminus of ubiquitin and specific cysteines of E2 and E3 enzymes.
• These bonds culminate in the formation of an isopeptide linkage between the activated carboxy terminus of ubiquitin and the epsilon-amino group of a lysine on a target protein or within another ubiquitin chain (usually K48 of ubiquitin) resulting in the generation of chains of ubiquitin (polyubiquitin or multiubiquitin) which may be from 4–20 ubiquitin moieties.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot (ECL): 1:5000Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
• Positive Controls:Rat Brain Tissue Extract and Heat-shocked HeLa Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.