84-1057-55　［取扱停止］Synaptotagmin I, p65 (Syt1) 50ug　S9109-20

特徴

• Synaptotagmin (also known as p65) is a synaptic vesicle protein that consists of an amino-terminal intravesicular domain, a single transmembrane domain, and a cytoplasmic domain composed of two repeats homologous to the C2 regulatory domain of protein kinase C.
• Synaptotagmin forms a homo-oligomer that binds calcium in a complex with negatively charged phospholipids.
• This observation, along with the fact that no synaptotagmin homolog has yet been identified in the constitutive secretory pathway, suggests that synaptotagmin may function as a calcium sensor that triggers exocytosis.
• The apparent low affinity for calcium and the multiple binding sites for calcium displayed by synaptotagmin are consistent with the properties expected of the calcium trigger for vesicle fusion.
• Support for a role of synaptotagmin in synaptic vesicle docking of fusion includes direct interactions with syntaxin and neurexin, and either direct or indirect interactions with N-type calcium channels.
• The interactions with these three presynaptic plasma membrane proteins could potentially position synaptotagmin, and the vesicle, in an optimal position to respond to calcium.
• Functional data supportive of a role for synaptotagmin in regulated secretion include the inhibition of secretion generated by microinjection of either synaptotagmin peptides into the squid giant synapse, or soluble synaptotagmin fragments and anti-synaptotagmin antibodies into PC-12 cells.
• However, genetic studies on PC-12 cells, as well as Drosophila and C.
• elegans, have demonstrated that synaptotagmin is not absolutely required for regulated neurotransmitter release.
• This may reflect the ability of another protein, such as rabphillin, to compensate partially for the loss of synaptotagmin.
• Alternatively, synaptotagmin may not be an essential component of the machinery required for vesicle docking and fusion, but rather may function as a calcium-sensitive negative regulator of the constitutive fusion machinery.
• This possibility is supported by recent electrophysiological recordings from synaptotagmin-deficient Drosophila, which revealed an increase in spontaneous neurotransmitter release and a decrease in evoked release.
• Applications:Western Blot (ECL): 1:1,000 ImmunoprecipitationImmunocytochemistryImmunohistochemistry: paraffinOptimal dilutions to be determined by researcher.
• Positive Controls:Rat Brain Tissue Extract
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.