84-1057-50　Superoxide Dismutase, Mn (SOD Mn) 50ug　S8060-10B

特徴

• Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide.
• There are three SOD isoenzymes in mammalian cells.
• They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2).
• The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol.
• Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix.
• Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria.
• Mn SOD has also been shown to be a tumor suppressor in human breast cancer.
• Overexpression of this enzyme protects neurons from NMDA-and nitric oxide-induced neurotoxicity.
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot (Colorimetric): 1:1000Flow Cytometry: 1:100Immunoprecipitation: 1:100Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.