p38 (HOG1), also known as SAPK-2, reactivating kinase or cytokine-suppressive binding protein, is a mouse 38 kD serine threonine kinase that belongs to the mitogen-activated protein kinase family (1).
p38 is identified as one of the major tyrosine-phosphorylated proteins in mouse monocyte cell lines upon lipopolysachharide (LPS) treatment (2).
Human homolog of p38 binds to cytokines suppressive anti-inflammatory drugs (CSAIDs), an anti-inflammatory drug that inhibits IL-1 and TNF-a synthesis (1).
HOG1, the yeast homolog of p38, is stimulated by osmotic shock (3).
p38 is activated by MAPK kinase MKK3 and Sek1/MKK4, the latter kinase is also involved in the activation of the stress-activated protein kinase pathway (4).
In LPS-treated monocytic cells, the activity of a transcription activators called myocyte-enhancer factor 2 (MEF2) is increased through p38-catalysed phosphorylation, which results in increased c-jun gene transcription (5).
p38 also activates MAPKAP kinase 2 in IL-1-stimulated cells (6).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin).
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1:10,000 Immunoprecipitation: 1:500 Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes human p38. Species Crossreactivity: monkey, mouse, rat, bovine, rabbit, porcine, canine, hamster, chicken, sheep and guinea pig.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Ca Ch Gp Hm Hu Mk Mo Po Rb Rt Sh
Immunogen：Synthetic peptide corresponding to a portion of human p38. The sequence is completely conserved in mouse.
