Rab5 is a 24kD GTP-binding protein that regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes.
It is localized to the cytoplasmic side of the plasma membrane, clathrin-coated vesicles, and early endosomes.
Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis.
The different guanine nucleotide binding states of Rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic.
Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion.
Studies using Rab5 mutant that hydrolysed xanthosine 5í-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion.
GTP hydrolysis by Rab5 is postulated to determine the frequency of membrane docking and fusion events.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1:1000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol
Specificity：Recognizes human Rab5 at ~26kD. Species Crossreactivity: mouse, rat, bovine, canine, guinea pig, hamster and monkey
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Bo Ca Gp Hm Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to amino acid portion of human Rab5
