Nuclear factor-kappa B, also known as NFkappaB, KBF1, p110 and EBP-1, was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes (1,2).
NF-kappaB is a heterodimer that consists of a 50kD DNA binding subunit (p50) and a 65 kD transactivation subunit (p65/Rel A) (3).
Both of these subunits exhibit sequence homology to the protooncogene c-Rel (4).
The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 (5,6) and p100 (7), respectively.
The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkappaB.
Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (interleukin-1, tumor necrosis factor), and viral infection, IkappaB is phosphorylated and degraded by proteosome.
The active NF-kappaB heterodimer is translocated into the nucleus and will induce gene expression.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot (Colorimetric): 0.5ug/ml.
Detects a band at~50kD and ~105kDOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in borate buffered saline, pH 8.2.
Specificity：Recognizes mouse NFkB. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to the sequence near the N-terminus of mouse NF-kB conjugated to KLH.
