MAP kinase kinase 6 (Mek6/MKK6) is a 38 kD dual-specificity protein kinase that is abundant in skeletal muscle and is activated by UV light, anisomycin, osmotic shock and inflammatory cytokines, but not by phorbol esters, NGF or EGF .
Mek6 phosphorylates and activates MAP kinases such as p38 and p38 beta .
Mek6 is phosphorylated and activated by ASK1, a MAPKKK that induces apoptosis when overexpressed.
Mek6 also interacts with mixed lineage kinase-3 (MLK-3), a kinase that participates in stress response kinase cascade, possibly by phosphorylating MKK4, an isoform of Mek6.
Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 5ug/mlImmunoprecipitation: 4ug/ml Western Blot (Colorimetric): 4ug/mlOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in borate buffered saline, pH 8.2, containing 50% glycerol and 0.09% sodium azide.
Specificity：Recognizes human MEK6. Antibody specificity is confirmed by peptide inhibition studies. Species Crossreactivity: rat, mouse and Xenopus
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt Xe
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment near the C-terminus of human MEK6, UniProt accession #P52564.
