Cells from many organisms increase their expression of a class of proteins known as heat shock or stress proteins in response to adverse changes in their environment.
The Hsp70 family of stress proteins includes a group of ~70kD highly conserved, ubiquitous proteins located within different intracellular compartments, and involved in cellular processes such as protein trafficking, folding, and prevention of aggregation.
Hsp70B’ is a variant of Hsp70 that originates from a separate gene, producing a protein more basic and with different stress-induction characteristics than Hsp701.
The Hsp70B’ gene shares 77% identity to Hsp70, and 70% identity to the constitutively expressed Hsc70.
Unlike Hsp70 which shows basal levels of expression and is induced by heat and various stressors, Hsp70B’ is strictly heat-inducible and shows no basal level expression2,3.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml Immunoprecipitation: 1:100Optimal dilutions to be determined by researcher.
Recommended Positive Controls:HeLa Heat Shocked Cell LysateHsp70B' Protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Hsp70B' at ~70kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the sequence near the C-terminus of human Hsp70B’.
