84-1056-89　［取扱停止］Heat Shock Protein 110 (HSP110) 100ug　H1833-05

特徴

• Hsp 110 (110kD) belongs to a family of large stress proteins referred to as the Hsp110/SSE family (1).
• The proteins in this family are the most distantly known relatives of the well studied Hsp70 family.
• Members of this group are significantly larger in size and contain sequences not present in members of the Hsp70 family.
• The mammalian protein has recently been cloned (1) and found to share 30–33% amino acid identity with members of the Hsp70 family, most of which occurs in the conserved ATP-binding domain of these molecules.
• Presently, all identified members of the Hsp70-like sequences can be categorized as being members of either one of these two groups; i.e.
• the DnaK (i.e.
• Hsp70) family or the Hsp110/SSE family (1).
• While most stress protein families (20kD, 60kD, 70kD, and 90kD) have been studied extensively, the Hsp110 species has been virtually ignored.
• Reports to date indicate that Hsp110 is a normal constituent of mammalian cells, it is induced by heat shock and its induction strongly correlates with the expression of thermotolerance (2).
• Furthermore, Hsp110 has been shown to be associated with the nucleolus (3) and Western blot analysis of several murine tissues indicates that Hsp110 is constitutively expressed at varying levels in all mouse tissues and is highly expressed in brain (1).
• Applications:Suitable for use in Immunoprecipitation and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1,000 (colorimetric)Optimal dilution to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.