HSF1 belongs to a family of Heat Shock transcription Factors (HSFs) that bind to highly conserved heat shock elements (HSEs) in the promoter regions of heat shock genes and regulate the expression of heat shock proteins (Hsps).
Multiple HSFs exist in higher eukaryotes whereas yeast and Drosophila have only a single HSF.
HSF1, HSF2, HSF3 have been reported in chicken, HSF1 and HSF2 in mouse and HSF1, HSF2, and HSF4 in human.
Most HSFs have several common functional motifs whose amino acid sequences are highly conserved.
At the N-terminus of all HSFs there is a DNA binding domain essential for binding to the HSE.
Hydrophobic repeats located adjacent to the DNA binding domain are essential for the formation of active trimers which enable all HSFs to specifically bind to DNA.
Another short hydrophobic repeat located towards the C-terminal region of most HSFs is thought to be necessary for suppression of trimerization except in the case of yeast HSF and human HSF4.
The mouse HSF1 exists as two isoforms which arise from alternative splicing of the HSF1 pre-mRNA.
On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.
Similar to HSF1, murine HSF2 also exists as two isoforms, the smaller HSF2-beta isoform and the longer HSF2-alpha which has been shown to be more transcriptionally active.
Constitutive binding of HSF2 to HSE has been found with spermatogenic cells post-implantation embryos and embryonal carcinoma cells in mouse and these observations suggest that HFS2 may have roles in differentiation and development.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot (Colorimetric): 1:1000Optimal dilutions to be determined by researcher
Positive Controls:Recombinant Human HSF-1 Protein (H1830-44)HeLa Heat Shocked Cell Lysate (No Recovery Time)Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species Crossreactivity: mouse, rat, monkey and rabbit
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rb Rt
Immunogen：Recombinant protein corresponding to human HSF-1 expressed in E. coli.
