84-1056-65　Glycogen Synthase Kinase 3 beta (GSK3b) 25ug　G8170-44F

特徴

• GSK3 beta, also known as Tpk1 (tau protein kinase-1), is a 47kD protein-serine/threonine kinase with broad specificity that phosphorylates and inactivates glycogen synthase, the phosphatase 1 inhibitor protein, and other proteins that feature a (S/T)PXX(Tp/Sp) recognition motif.
• It is ubiquitously distributed, but highly expressed in mammalian brain, and occurs in two isoforms, 51kD (alpha) and 47kD (beta).
• This monomeric proline-directed kinase is inhibited when it is phosphorylated by protein kinase B and S6 protein kinases.
• It is activated when it is phosphorylated by an unknown protein-tyrosine kinase.
• GSK3beta is inhibited in response to insulin.
• GSK3beta is phosphorylated and inactivated by protein kinase C, p70, S6 kinase and Rsk-1.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot (Colorimetric): 1:1000 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by researcher.
• Positive Controls:HeLa Cell Lysate, Mouse Brain Tissue Extract Rat Brain Tissue Extract
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.