84-1056-64　［取扱停止］Glucose Regulated Protein 94 (Grp94, gp96) 100ug　G3057-42

特徴

• Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which belongs to the Hsp90 family of molecular chaperones.
• Grp94 possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal thought to lead to the retention of proteins in the pre-Golgi compartments (1).
• Grp94 expression is upregulated by stress conditions which promote protein misfolding or unfolding (2).
• In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides (3,4).
• Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation.
• Hsp90 and Grp94 may differ in their nucleotide binding properties.
• The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol.
• .
• Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:80Optimal dilutions to be determined by researcher.
• Positive Controls:Mouse Brain Tissue Extract or Rat Brain Tissue Extract
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.