Fas/APO-1/CD95 is a 36kD cell surface type I-membrane glycoprotein with an apparent molecular weight of 44kD on SDS-PAGE (1).
Fas is a member of the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40.
Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells (2,3).
Both Fas and TNFR contain a 70 amino acids cytoplasmic ìdeath domainî that is responsible for transmitting signal for apoptosis (4).
Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long form of FLIP (5,6).
Insertion of an early transposable element in intron 2 of Fas structural gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting that Fas is involved in clonal deletion of autoreactive T cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1:1000Immunohistochemistry: 1:50Optimal dilutions to be determined by researcher.
Positive Control:Rat Brain Tissue Extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Recognizes mouse Fas at ~45kD. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a portion of mouse Fas.
