EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms.
The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha.
Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis (1).
Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses (2).
EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52 kD) (3).
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot (ECL): 1ug/ml Optimal dilutions to be determined by researcher.
Positive Controls:HeLa Cell Lysate: Use 20ug per lane.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes a ~36kD protein, corresponding to the apparent molecular mass of rat eIF-2alpha. Species Crossreactivity: human, mouse, bovine, canine, guinea pig, hamster, monkey, porcine, rabbit and sheep.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Bo Ca Gp Hm Hu Mk Mo Po Rb Rt Sh
Immunogen：Synthetic peptide 13aa of Rat eIF-2alpha phosphorylated (KLH).
