Dehydrins are a family of proteins that become abundant during dessication in seedlings and embryos of cereal crop plants including barley, corn, wheat and rice.
Similar proteins have been found to accumulate regardless of whether the environmental cue is evaporation, increased solutes, embryo desiccation, low temperature or treatment with ABA (abscisic acid–a plant growth regulator).
Dehydrin proteins have a highly conserved lysine-rich block (KIKEKLPG) found near the carboxy terminus which may be present in one or more copies in the protein.
This sequence appears to be characteristic of and unique to dehydrins.
Dehydrins have been observed in a wide range of Angiosperms, and possibly all plants can synthesize dehydrins.
There is also evidence that a cyanobacterium can synthesize a 39kD dehydrin during osmotic stress.
The number and size of dehydrins varies widely between species, ranging from 15-150kD.
Recent studies have shown that the cereals have larger families of dehydrins than had been previously described.
For example, at least 10 dehydrin polypeptides have been detected in Himalaya barley ranging in apparent molecular mass from 16 to 80 kD.
Additional differences have been found in the relative quantity of individual dehydrin species depending on the location within the seedling.
Furthermore, there are striking differences both in the number and size of dehydrins between barley cultivars, suggesting that the total number of barley dehydrin alleles is vast.
It should be noted that dehydrins have often been found to migrate 3-5kD greater than the predicted molecular weight based on the amino acid sequence.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot (Colorimetric): 1:1000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes plant dehydrin at ~65kD. Species Crossreactivity: algae, barley, corn, cotton, cowpea, cucumber, ferns, Ginko, grass, moss, peanut, pine, radish, rice, soy, tomato and wheat.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide corresponding to a sequence near the C-terminus of Dehydrin, conjugated to BSA. The sequence is identical to barley, rice, maize and wheat. (Y00842)
