84-1056-53　［取扱停止］Cytochrome P450 Reductase 50ug　C9095-24

特徴

• NADPH-cytochrome P450 reductase is a microsomal component that is essential for transferring reducing equivalents from NADPH to the microsomal heme oxygenase isozymes, HO-1 and HO-2, to the various cytochrome P450 isozymes and to cytochrome c and b5.
• The reductase is inducible in the liver, phenobarbital and 3-naphthoflavone are good inducers of the enzyme.
• The rat enzyme has an apparent molecular weight of 78kD and contains both FAD and FMN.
• Nitric oxide synthase is the other known enzyme that also contains FAD and FMN.
• The reductase has been isolated from different species and a close range of apparent molecular weights of 76 to 81kD.
• The amino acid sequence of the reductase from several species has been deduced from cDNA.
• The enzyme has a short hydrophobic N-terminus that serves to anchor the reductase to the endoplasmic reticulum and nuclear envelope; the reductase without the hydrophobic portion retains its ability to transfer electrons to cytochrome c, but not to cytochrome P450.
• The 70kD hydrophilic region contains the flavin and NADPH binding domains.
• The purified enzyme in the presence of a detergent, such as Triton X-100 (0.02%), can reconstitute heme degradation activity of purified or expressed heme oxygenase isozymes(1,3–8).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot (Colorimetric): 1:2000Optimal dilutions to be determined by researcher.
• Positive Controls:Rat Liver NADPH Cytochrome P450 Reductase Protein (C9095-23)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.