Ethanol-inducible cytochrome P450 IIE1 (CYP2E1) is considered one of the major causes of oxidative stress in the liver following ethanol treatment.
Radicals produced by this enzyme are thought to induce lipid peroxidation reactions and other damage to the cells and to be a major cause for ethanol-dependent liver toxicity.
The importance of CYP2E1 lies in its ability to metabolize a wide range compounds such as organic solvents, acetaminophen, dimethylnitrosamine and aliphatic alcohols, which have relevant toxicological effects in humans.
To date, over 75 substrates of CYP2E1 have been identified.
CYP2E1 is mainly found in the liver although it has also been detected in a variety of other organs, including the brain, colon and lungs.
In the liver it is localized to the centrilobular region, specifically the hepatocyte layers most proximal to the central vein.
Cytochrome P450 is considered to be part of the mixed function oxidase (MFO) sytem along with NADPH-cytochrome P450 reductase.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat Cytochrome P450 at~50-55kD. Inhibits microsomal benzene metabolism. Species Crossreactivity: human, mouse, canine, monkey, guinea pig, rabbit, clam and equine.
