Cytochrome c is an electron transport protein from mitochondria.
A primary means of caspase activation by p53 is through induction of cytochrome c release from mitochondria.
Cleavage of Bid, a Bcl 2 family member, by caspase-8 produces a truncated tBid molecule which triggers mitochondria release of cytochrome c which binds to the adaptor protein Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex.
This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot (Colorimetric): 1:5000 Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.08% sodium azide.
Specificity：Recognizes human Cytochrome C at ~14kD on Western Blots. Species crossreactivity: rabbit, rat and canine.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Ca Hu Rb Rt
Immunogen：Recombinant protein corresponding to full length human Cytochrome C.
