Alpha-crystallins composed of alpha-A (~19kD) and alpha-B (~19.2kD) subunits, are major water-soluble proteins accounting for almost 50% of the total protein of the mammalian transparent eye lens and they are also found in a variety of other tissues.
The alpha-crystallins have sequence homology as well as structural and functional similarities with the small heat shock proteins such as Hsp25.
The alpha-crystallins and Hsp25 have been shown to act as molecular chaperones by suppression of heat-induced aggregation of other proteins and are efficient inhibitors of elastase.
The alpha-B subunit like Hsp25 was also found to be a substrate for protein cross-linking by tissue-type transglutaminase.
The alpha-crystallins may be involved in metabolic pathways important for the development, maintenance, or pathology of the lens.
They prevent destabilized proteins from precipitating out of solution by forming a soluble high-molecular-weight (HMW) complex.
The bovine HMW protein form of the lens is composed mostly of alpha-crystallin in a highly aggregated state which has markedly reduced chaperone ability compared to alpha-crystallin.
NMR spectroscopy of the naturally occurring HMW complex shows that the short C-terminal extension of alpha-B subunit in HMW has lost its flexibility whereas the alpha-A subunit extension is still flexible.
These results suggest that depletion of alpha- crystallin with age as it is converted to HMW, could be one of the contributing factors to cataract formation in the lens.
Applications: Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1:1000Optimal dilutions to be determined by researcher.
Positive Control:alpha Crystallin Protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.0, 3% BSA, 0.09% sodium azide, 50% glycerol
Specificity：Recognizes rat Crystallin, alpha B, phosphorylated (Ser59). Species Crossreactivity: mouse, bovine, porcine and sheep.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to aa54-64 of human Crystallin, alpha B, phosphorylated at Ser59.
