Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye.
Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function.
Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (paraffin sections).
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, colorimetricOptimal dilutions to be determined by the researcher.
Positive Control:Bovine alphaB-Crystallin Protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Crystallin, alpha B. Species Crossreactivity: rat, mouse, bovine and porcine. There is no reactivity with beta-crystallin nor alpha-A-crystallin.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to the sequence near the C-terminus of human αB-Crystallin. The sequence is completely conserved in rat, rabbit, and bovine.
