Alpha-crystallin is one of the major structural proteins in the vertebrate eye lenses (2).
It occurs as large aggregrates of up to 106D composed of two gene products, alphaA-and alphaB-crystallin.
These two subunits have a sequence homology of approximately 57%.
In humans, the alphaA-crystallin gene encodes a polypeptide of 173 residues and the alphaB-crystallin gene encodes a 175 residue polypeptide.
They are related to the small heat proteins (sHspís) (3).
The alphaB-crystallin protein, like sHspís, is induced by heat and osmotic shock (4).
In addition, alpha-crystallin and sHspís protect other proteins against heat-induced aggregation (6).
The two other crystallin families, ß and gama, are homologous to each other but not to the alpha family or the sHspís.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot (Colorimetric): 1:1,000 Optimal dilutions to be determined by researcher.
Positive Controls:Bovine alpha-Crystallin ProteinBovine alphaA-Crystallin ProteinBovine alphaB-Crystallin Protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human αA- and αB-Crystallin at ~20kD. Species Crossreactivity: mouse and bovine. There is no reactivity with beta-crystallin.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Bo Hu Mo
Immunogen：Synthetic peptides EEKPTSAPSS and REEKPAVTAAPKK based on the human alphaA crystallin (residues 164-173) and the human alphaB crystallin (residues 163-175) respectively (6), was synthesized and the peptide coupled to KLH.
