Chaperonin 10 (Cpn 10 or Hsp10), is the ~10kD mammalian equivalent to GroES of E.
coli.
Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins.
Chaperonin function in vivo requires two distinct family members, cpn60 and cpn10, both of which are also present in chloroplasts and mitochondria.
The best studied cpn60 and cpn10, are the GroEL and GroES proteins of E.
coli, respectively.
Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly processes.
Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum.
It has been reported that human cpn10 is identical with early pregnancy factor (EPF), which is involved in control over cell growth and development.
This identification suggests that cpn10 may act like a hormone in stressful situations such as pregnancy
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1:2000Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by the researcher.
Positive Controls:Heat Shocked HeLa Cell Lysate, C3450 Chaperonin 10, Recombinant, Human (cpn10, Heat Shock Protein 10, Hsp10)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid, in PBS, pH 7.2, 0.09% azide, 50% glycerol.
Specificity：Recognizes human Chaperonin 10 at ~10kD by Western blot. Species Crossreactivity: Mouse, Rat, Bovine, Canine, Guinea pig, Porcine, Rabbit, Sheep and Xenopus
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Ca Gp Hu Mo Po Rb Rt Sh Xe
Immunogen：Synthetic peptide corresponding to the sequence near the C-terminus of human Cpn10.
