Casein kinase II (CK2), a serine/threonine protein kinase, is a holoenzyme (130kD) comprised of two alpha subunits (catalytic) and two beta (regulatory) subunits (2).
The alpha subunit may be present as either the 45kD alpha subunit or as the 41kD alpha’ isoform.
CK2 phosphorylates many proteins in vivo, on serine or threonine residues that are N-terminal, to acidic amino acids such as in casein.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 4ug/ml (ECL)Immunohistochemistry: 1.7-8.5ug/ml Optimal dilutions to be determined by researcher.
Positive Control: HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in BBS, pH 8.2, 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt).
Specificity：Recognizes ~45kD and ~41kD proteins, corresponding to the apparent molecular masses of CK2 alpha and CK2 alpha' on SDS-PAGE immunoblots. Species Crossreactivity: human, mouse, rat, bovine, fish (chinook salmon), Drosophila, guinea pig, monkey, rabbit and sheep. An additional band with an apparent molecular mass of ~38kD is detected in brain and heart tissue extracts. Species sequence homology: alpha subunit of CK2 from rat, mouse, rabbit, chicken, Xenopus, C. elegans, and zebrafish.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Bo Dr Gp Hu Mk Mo Rb Rt Sh
Immunogen：Synthetic peptide corresponding to a portion of human CK2α (casein kinase 2α).
