Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen, but also found in other membrane-bound organelles, the cell surface and extracellularly.
Reports of nuclear localization are likely immunostaining artifacts (7).
CRT is also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein) (7,8).
It contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+-binding protein.
CRT is comprised of three domains; a 180 residue N-terminal domain, a proline-rich P-domain (residues 189–288) that binds Ca2+ with high affininty and has homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (7,9).
The P-domain may interact with the cochaperone ERp57 (Grp58), a thiol reductase.
The NMR structure of the P-domain (residues 189–288) is an extended hairpin that seems to form an extended, somewhat curved protrusion from the CRT core domain (9).
Both CRT and its membrane bound homolog, CNX, interact with proteins and glycoproteins that have monoglucosylated N-glycans (7,9,10).
The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex (9,10).
Other functions atttributed to CRT include adhesion, blood function, cardiac and neuronal development gene expression and other diverse functions.
CRT is also implicated as an autoantigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block and halothane hepatitis (7).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1,000, human; 1:500, mouse; ECLOptimal dilutions to be determined by the researcher.
Positive Controls:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Calreticulin at ~63kD. Species Crossreactivity: bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the sequence near the C-terminus of human Calreticulin.
