84-1056-25　Calnexin 200ug　C1035-26C

特徴

• Calnexin (CNX), an abundant ~90kD molecular chaperone, is a unglycosylated resident ER transmembrane protein.
• In mammalian cells, calnexin, together with calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans (4).
• Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded.
• Calnexin associates with numerous oligomeric protein complexes within the ER, including beta1 and alpha6 integrins (5), major histocompatibility class I (6, 7) and class II molecules (8), the antigen receptors expressed on T and B lymphocytes (7), human thyroperoxidase (hTPO) (9), and acetylcholine receptor (10).
• Recent data also suggest that calnexin might be responsible for the prolonged retention of pro-alpha6 integrin within ER compartment (11).
• Furthermore, calnexin has been demonstrated to function as a bonafide molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins (12).
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot (Colorimetric): 1:1,000 Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by researcher.
• Positive Control:Heat Shocked HeLa Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.