Calcineurin is a Ca2+/Calmodulin-dependent serine/threonine protein phosphatase.
The active enzyme is a heterodimer consisting of a catalytic A subunit and a myristylated regulatory B subunit that binds calcium.
Calcineurin, which is the target of immunosuppressant drugs such as cyclosporin A and FK506, controls the translocation of Nuclear factors of activated T-cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T-cells.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot (ECL): 1:1000Optimal dilutions to be determined by researcher.
Positive Controls:Rat Brain Tissue Extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes an~61kD protein, corresponding to the apparent molecular mass of human calcineurin A. Species Crossreactivity: mouse, rat, rabbit, canine, Drosophila, hamster, bovine, chicken, monkey, porcine and sheep.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Ca Ch Dr Hm Hu Mk Mo Po Rb Rt Sh
Immunogen：Synthetic peptide corresponding to human calcineurin A.
