Cleavage of heme b (Fe-protoporphyrin IX) at the alpha-methene carbon bridge to form the open tetrapyrrole, biliverdin IXalpha and carbon monoxide (CO) is catalyzed by heme oxygenase (HO) isozymes HO-1 and HO-2 (heme hydrogen-donor: oxygen oxidoreductase; EC 1.14.99.3).
In mammalian species biliverdin reductase (BVR; bilirubin: NAD(P)+ oxidoreductase; EC 1.3.1.24) converts the open tetrapyrrole to bilirubin.
This pathway is the only efficient way of making bilirubin and hence deterring activation of oxygen by the heme molecule.
The two isozymes of heme oxygen, one of which is referred to as HO-1, is a member of the heat shock protein family (Hsp32), and the second, or HO-2, is a constitutive form and is expressed at exceedingly high levels in the brain and testes.
The end products of the heme degradation process have important physiological activities.
CO is suspected of being a potential messenger in the brain and systemic organs wherein by interacting with the heme-dependent form of guanylate cyclase it stimulates cGMP-production.
Bile pigments have been demonstrated to display potent antioxidant activity and have effective antiviral activity against HIV and herpes virus.
BVR is unique among all enzymes characterized to date in having two pH optima (6.8 and 8.7), using a different cofactor at each pH range (NADH at pH 7.0 and NADPH at pH 8.7).
The enzyme displays pI and molecular mass microheterogeneity which appear to be a result of post translational modifications.
In rat the enzyme also shows a tissue specific developmental pattern.
BVR is not a inactivated by heat shock and its preexisting message is not sequestered from translation subsequent to thermal stress.
Applications:Suitable fror use in Western Blot and Immunohistochemistry.
Optimal dilutions to be determined by researcher.
Recommended Dilutions:Western Blot: 1ug/ml, detects bands of ~33kD (rat) and ~41-42kD (human)Optimal dilutions to be determined by the researcher.
Positive Controls:Recombinant Rat Biliverdin Reductase Protein
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes a 41-42kD protein (human) and 33kD protein (rat), corresponding to the apparent molecular mass of biliverdin reductase on SDS-PAGE immunoblots. Species Crossreactivity: hamster, porcine and mouse (weak)
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hm Hu Po Rt
Immunogen：Recombinant protein corresponding to rat Biliverdin Reductase.
