84-1056-18　Bax (BCL2-associated X protein, Apoptosis regulator BAX, cytoplasmic isoform beta, Apoptosis regulator BAX, membrane isoform alpha, BAX protein, cytoplasmic isoform delta, BAX protein, cytoplasmic isoform gamma, BCL2L4) 200ug　B0175-04C

特徴

• Bax is a 21kD protein that was identified as a protein that associates with Bcl-2, a repressor of apoptosis.
• Bax is a member of the bcl-2 family of proteins and contains Bcl-2 homology regions (BH-) 1, 2 and 3.
• Alternative splicing of Bax mRNA results in a 21kD membrane (alpha) and two forms of cytosolic proteins (beta and gama).
• Unlike Bcl-2, Bax initiates apoptosis when it homodimerizes or when it fails to form heterodimers with Bcl-2.
• Overexpression of Bax increased the rate of apoptosis caused by cytokine deprivation in an IL-3-dependent manner.
• Bax expression is stimulated by transcriptional activator p53, a tumor suppressor protein that induces apoptosis caused by damage to DNA.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot (ECL): 1:1000Optimal dilutions to be determined by researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.