Bak is a 23kD homolog of the apoptosis inhibitor, Bcl-2 with an apparent molecular weight of 30kD on SDS-PAGE.
Bak is a member of the Bcl-2 family of proteins and contains the bcl-2 homology regions (BH-) 1, 2 and 3.
Unlike Bcl-2, Bak promotes apoptosis by interacting with and counteracting the anti-cell death effect of Bcl-2.
Enforced expression of Bak induces rapid and extensive apoptosis of serum-deprived fibroblasts (1).
However, Bak can inhibit cell death in an Epstein-Barr virus transformed cell line (2).
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Western Blot (ECL): 1:1000Immunoprecipitation: 1:100Immunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Positive Controls:HeLa Heat Shocked Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Bak at ~30kD. Species Crossreactivity: hamster, monkey and porcine
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hm Hu Mk Po
Immunogen：Synthetic peptide corresponding to aa2-14, ASGQGPGPPRQEC, of human Bak conjugated to KLH.
