It exhibits sequence homology to Bcl-2, a protein that prevents apoptosis, within its BH1 and BH2 domains (1).
Bad heterodimerizes with Bcl-Xl and Bcl-2 and reverses the apoptosis suppression effect by Bcl-Xl but not Bcl-2.
Bad is phosphorylated by Raf-1, a serine-threonine kinase that is targeted to the mitochondrial membranes by Bcl-2 (2).
In the presence of survival factor IL-3, Bad is phosphorylated on two serine residues within its 14-3-3 consensus binding sites.
Phosphorylated Bad is then sequestered in the cytosol by binding to 14-3-3 and unable to heterodimerize with Bcl-Xl on mitochondrial membranes to promote apoptosis (3).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot (ECL): 1ug/ml Immunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Tissue Extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide., 50% glycerol.
Specificity：Recognizes mouse BAD at ~23kD. Species Crossreactivity: monkey, rat, hamster, bovine, sheep, porcine, canine (weak), rabbit, chicken (weak) and Drosophila. This 23kD band can be specifically inhibited in peptide competition studies.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Dr Hm Mk Mo Po Rb Rt Sh
Immunogen：Synthetic peptide corresponding to amino acids 19-35 of mouse BAD (KLH).
