Apoptosis inducing factor (AIF) is a mitochondrial inter-membrane flavoprotein that is ubiquitously expressed both in normal tissues and in a variety of cancer cell lines.
AIF is synthesized as a nonapoptogenic precursor in the cytoplasm and efficiently imported into the mitochondrial inter-membrane space.
Mature AIF protein is normally confined to the mitochondrial inter-membrane space.
In a variety of different apoptosis-inducing conditions AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus.
Extra-mitochondrial AIF induces nuclear chromatin condensation and large scale (~50kb) DNA fragmentation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-1ug/ml, detects a band of ~67kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human AIF. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a portion of human AIF.
