84-1056-11　［取扱停止］4E Binding Protein 2 (4E-BP2, eIF4E-BP2, PHAS-2) 100ug　0004-30

特徴

• PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation (1).
• PHAS-I and ñII were found to have overlapping but different patterns of expression in tissues.
• PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found (2).
• The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF-4E and blocking translation by preventing access of eIF-4G to the 5í cap of the mRNA (1).
• Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1.
• Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation (1).
• Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro.
• However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II.
• Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and ñII suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation (1).
• Applications:Immunohistochemistry: paraffinWestern Blot (Colorimetric): 4ug/ml Western Blot (ECL): 4ug/ml Optimal dilutions to be determined by researcher.
• Positive Controls:Rat Brain Tissue Extract
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.