Metabotropic glutamate receptors have been classified into three groups.
In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission.
Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC).
Class II and class III mGluRs localize to the presynaptic membrane and are negatively coupled to adenylate cyclase.
It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immuncytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml (ECL) on rat brain microsomal preparation.
Detects a band of ~132kD
Immunohistochemistry: 0.1-0.2ug/ml.
Rat and mouse brain sections fixed with 0.1M PBS, pH 7.4, 4% paraformaldehyde, 15% saturated picric acid.
Slide-mounted tissue sections were processed for indirect immunofluorescence.
Slides were incubated with blocking buffer for 1 hour at RT.
M3884-79D was diluted with blocking buffer to the appropriate working concentration.
Blocking buffer was removed.
Slides were incubated overnight at 4ºC with diluted M3884-79D.
Slides were rinsed 3 times.
Slides were incubated with secondary antibodies for 1 hour at RT.
Slides were again rinsed 3 times and coverslipped.
Staining was examined using fluorescence microscopy
Immunocytochemistry: 0.5ug/mlImmunofluorescence: IndirectOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Specificity：Recognizes rat metabotropic glutamate receptor 5 (mGluR5). Demonstrates very little or no crossreactivity with mGluR1a. Species Crossreactivity: human and mouse.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to rat C-terminus mGluR5 (Metabotropic Glutamate Receptor).
