Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: IndirectImmunohistochemistry: 1:7500 to detected D-Alanine immunoreactive cell bodies located in the lateral and ventro-lateral thalamus of paraformaldehyde/glutaraldehyd fixed and frozen rat brain
Optimal dilutions to be determined by the researcher.
Specificity:Using a conjugate D-Alanine-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:CompoundCross-reactivity ratio (a)D-Alanine-PC1L-Alanine-PC1/> 50,000Glycine-PC1/> 50,000D-Valine-PC1/> 50,000D-Leucine-PC1/> 50,000(a) : D-Alanine-PC concentration/other conjugated amino acid concentration at half displacement;
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Purified
Purity：Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity：Recognizes protein conjugated D-Alanine. See additional crossreactivity data.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic D-Alanine conjugated to a protein carrier
