Microfibril-associated glycoprotein (MAGP) is a major structural component of connective tissue microfibrils.
MAGP-1 produced by keratinocytes may play some functional role in the formation of dermal matrix organization in the dermis.
Applications:Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:2000Western Blot: 1:200-1:500Immunohistochemistry: 1:50-1:100Immunoprecipitation: 1:100-1:200Optimal dilutions to be determined by the researcher.
Etching to Enhance Antigenicity:In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes.
After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 40% glycerol.
Specificity：Reacts with bovine MAGP1. No crossreactivity with MAGP1 from other species.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Bo
Immunogen：Protein fragment containing the front half of bovine MAGP1.
