84-1055-97　Fibrillin 2, exon 10, Gly-rich Domain 250ul　F4195-10

特徴

• Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules.
• Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb).
• Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule.
• Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2.
• The fibrillins are found throughout the connective tissue as integral components of extended fibrils.
• There is an aberrant expression of fibrillin-1 in Marfan syndrome.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:200-1:500Immunohistochemistry/Immunocytochemistry: 1:50-1:100ELISA: 1:1000-1:2000Immunoprecipitation: 1:100-1:200Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes.
• After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.