Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment.
Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations.
PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 5-10ug/mlImmunocytochemistry: 5-10ug/mlOptimum dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate fractionation.
Form：Supplied as a liquid in PBS, 0.08% sodium azide.
Specificity：Recognizes Protein Serine/Threonine phosphatase 2A/B alpha (55kD). Does not crossreact with other protein phosphatase isoforms. Species Crossreactivity: human, mouse, rat and bovine.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa14-27, NH2-Phe-Ser-Gln-Val- Lys-Gly-Ala-Val-Asp-Asp-Asp-Val-Ala-Glu-COOH, conjugated to KLH.
