Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, fat synthesis by adipocytes, and many others.
PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986).
In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals.
The PKC isoforms can be grouped based on their requirements for Ca2+ and phospholipids for activation.
The isoforms of group 1 include PKCa, PKC bI, PKC bII, and PKCg.
Group 1 isoforms require both Ca2+ and phospholipids for activation.
The isoforms of group 2 include PKC d, PKC e, PKC n, and PKCt.
Group 2 isoforms require phospholipid for activation, but do not require the presence of Ca2+.
The isoforms of group 3 include PKCs, PKC m, and PKC t/l.
Group 3 isoforms are not activated by the presence of diacylglycerol or by phorbol esters such as phorbol myristate acetate.
Applications:Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:250.
Using alkaline phosphatase or HRP
Immunohistochemistry (fresh or frozen sections): 1:250.
Note:Paraffin embedded or fixed tissues have not been tested
Immunocytochemistry: 1:250Note: With a more sensitive detection system such as enhanced chemiluminescence or ELISA, the antibody may be diluted further
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes rat PKC zeta. Species sequence homology: most mammalian.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ma Rt
Immunogen：Synthetic peptide corresponding to a distinct C-terminal region aa579-592, EYINPLLLSAEESV, from rat protein kinase C zeta.
