Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, and fat synthesis by adipocytes.
PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986).
In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals.
The PKC isoforms can be grouped based on their requirements for Ca2+ and phospholipids for activation.
The isoforms of group 1 (classical or conventional PKC’s) include PKC alpha, PKC betaI, PKC betaII, and PKC gamma.
Group 1 isoforms require both Ca2+ and phospholipids for activation.
The isoforms of group 2 (the novel PKC’s) include PKC delta, PKC epsilon, PKC eta, and PKC theta.
Group 2 isoforms require phospholipid for activation.
They do not require the presence of Ca2+.
The isoforms of group 3 (the atypical PKC’s) include PKC zeta, PKC mu and PKC tau/lambda.
Group 3 isoforms are not activated by the presence of diacylglycerol or by phorbol esters such as phorbol myristate acetate.
PKC eta is expressed in several tissues, including lung, skin, heart, neural tissues and epithelial tissues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 using enhanced chemiluminescence.
Note: If using a less sensitive detecting system such as alkaline phosphatase, decrease the dilution of the antibody
Optimum dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as liquid serum containing 0.05% sodium azide.
Specificity：Recognizes protein kinase C eta from most mammalian species including rodent and human.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Ma Rd
Immunogen：Synthetic peptide corresponding to aa666-683 (NQDEFRNFSYVSPELQP), a distinct C-terminal region from rat protein kinase C eta.
