Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, fat synthesis by adipocytes.
PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986).
In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals.
The PKC isoforms can be grouped based on their requirements for Ca2+ and phospholipids for activation.
The isoforms of group 1 (classical or conventional PKC’s) include PKC alpha, PKC betaI, PKC betaII, and PKC gamma.
Group 1 isoforms require both Ca2+ and phospholipids for activation.
The isoforms of group 2 (the noval PKC’s) include PKC delta, PKC epsilon, PKC eta, and PKC theta.
Group 2 isoforms require phospholipid for activation.
They do not require the presence of Ca2+.
The isoforms of group 3 (the atypical PKC’s) include PKC zeta, PKC mu and PKC tau/lambda.
Group 3 isoforms are not activated by the presence of diacylglycerol or by phorbol esters such as phorbol myristate acetate.
PKC eta is expressed in several tissues, including lung, skin, heart, neural tissues and epithelial tissues.
Applications:Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:250 using alkaline phosphatase or HRP
Immunohistochemistry (Fresh or frozen): 1:250Immunocytochemistry: 1:250Note: With a more sensitive detection system such as ECL, the antibody may be diluted further.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide
Specificity：Recognizes rat protein kinase C eta.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ma Rt
Immunogen：Synthetic peptide corresponding to aa672-683 (NH2-RNFSYVSPELQP) within a distinct C terminal region from rat protein kinase C eta. Species Sequence Homology: most mammalian.
