Applications:Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Fresh or frozen sections): 1:250.
Paraffin embedded and fixed tissues have not been tested
Immunocytochemistry: 1:250Western Blot: 1:250 using alkaline phosphatase or HRPWestern Blot: >1:250 using ECLELISA: >1:250Optimum dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a liquid containing 0.05% sodium azide.
Specificity:Recognizes rat PKCa. Expected to crossreact with most mammalian species based on known homologies.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Ma Rt
Immunogen:Synthetic peptide corresponding to a distinct C-terminal region, (SYVNPQFVHPILQSAV resides 657-672) from rat protein kinase C alpha.