84-1055-47　Prostaglandin E Synthase (PGE Synthase) 100ul　P9053-28

特徴

• The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins.
• The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation, and inflamatory and neoplastic diseases.
• The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2.
• Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified.
• COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets.
• COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids.
• The overexpression of COX-2 also leads to tumerogenesis.
• Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported.
• Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized.
• The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity.
• The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3).
• This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 with ECL.
• Incubate with P9053-28 for 2 hours, secondary antibody for 1 hour.
• Exposure time is 45 seconds.
• For alkaline phosphatase detection use lower dilutions.
• Extensive washing of transfer membrane is required to reduce non-specific background binding effects
• Optimum dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.